Composite
Part:BBa_K3286009
Designed by: Paul Alexander Niederau Group: iGEM19_Wageningen_UR (2019-10-16)
AcrIIA4 expression cassette
The AcrIIA4 is under control of the inducible L-rhamnose promoter. The AcrIIA4 expression was adapted by the use of different RBS sites.
The deeper analysis on varying Acr levels on inhibitory activity of dCas9 were assessed here: Part:BBa_K3286010
L-rhamnose inducible promoter: Part:BBa_K914003
AcrIIA4: Part:BBa_K3286000
bi-directional terminator: Part:BBa_K3286006
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |